Pesquisa e inovação biomédica avançada

The differential expression analysis of angiogenesis associated gene regulated breast cancer stem cells: A bioinformatics landscape

Neda Darbanrezaei1, Sahar Samieyan dehkordi2, Arsalan Jalili3, Romina Mehtar Araghinia1 and Amin Ebrahimi Sadrabadi4

Angiogenesis is the creation of new blood arteries from the arteries that play role in tumour development. Breast cancer is a common cancer in women and 58% of deaths associated with this type of cancer occur in less developed countries. Despite significant improvement in early diagnosis, breast cancer death rates remain high yet. The study of molecular mechanisms of angiogenesis is necessary for developing new diagnostic and therapeutic strategies for breast cancer. For more accurate analysis of correlation between angiogenesis and various pathways involved in the angiogenesis process, GEO dataset and ultimately selected 13 breast tumour and control samples has been used for analysis. Biojupies database has been used for in-depth analysis of a selected dataset. The down regulated genes in the breast cancer cells presented the differential expression which confirmed by volcano plot. Gene ontology (GO) analysis was performed on down regulated genes. The biological process (BP) analysis manifests the multiple biological roles for each gene. The cellular component (CC) analysis demonstrated the location where the protein/gene performs its molecular function. This localization led to understand the correlation between the component of the plasma membrane and down regulation of specific genes which manages angiogenesis process. The protein-protein interaction (PPI) network of down regulated genes was constructed by STRING. It concluded that TEK and KDR have the most significant correlation with other genes and Caveolin-1 and Endomucin are co-expressed, and their meaningful correlation with TEK and KDR is entirely obvious. The down regulation of selected genes on tumour cells led to expanding the angiogenesis process