Jornal de Oncologia Clínica e Experimental

Anticarcinogenic Effect ofEpigallocatechin-3-Gallate (EGCG) in Renal Carcinoma Induced by Aristolochic Acid in Male Rats

Sohail Hussain*, Mohammed Ashafaq, Rahimullah Siddiqui, Khaled Hussain Khabani, Ahmad Suliman Alfaifi and Saeed Alshahrani

Introduction: To elucidate the chemopreventive effect of Epigallocatechin-3-Gallate (EGCG) a polyphenol found in green tea having antioxidative, anticancer, anti-inflammatory, anticollagenase, and antifibrosis properties property against carcinogenic effect of Aristolochic Acids (AA) extract from a Chinese herb Aristolochia species (Aristolochia and Asarum) in wister male rats. Material and Method: The consequences of EGCG a dose (20 mg/kg b.w.) for 30 days was assessed against renal carcinoma induced by AA after a dose of (20 mg/kg b.w.) for 90 days. The biochemical markers (Urea, Uric acid and Creatinine), imbalance in oxidative parameters (MDA, GSH, Catalase and SOD), caspases 3,9 expression and histopathological studies were determined for quantifying the carcinogenic effect of AA. Results: Treatment of AA results significant increase in free radicals, serum markers, caspases 3 and 9 activity and histopathological changes. After co-treatment with EGCG markedly reverse all the parameters. Furthermore, it also restores the activity of antioxidative enzymes. Conclusion: Our study shows, that carcinogenic property of AA is restore by treating with EGCG in renal tissue.