Revista de Gestão da Biodiversidade e Silvicultura

Influence of Extraction Methods on the Composition of Essential Oils of Achillea millefolium L. from Lithuania

Hanen Marzouki, Alessandra Piras, Silvia Porcedda, Danilo Falconieri and Edita Bagdonaite

Influence of Extraction Methods on the Composition of Essential Oils of Achillea millefolium L. from Lithuania

In this study, flowering aerial parts of Achillea millefolium were used as a matrix for supercritical CO₂ extraction (SFE) of volatile oil. The collected extracts were analyzed by GC-FID and GC-MS methods and their composition were compared with that of the essential oil isolated by hydrodistillation (HD). The composition of the essential oil obtained by hydrodistillation and SFE methods is widely different. Indeed, the SFE volatile oil had a pale yellow color whereas the HD oil had a blue color due to the presence of chamazulene (48.0% vs. 4.3%). Other important constituents of HD oil were (E)- caryophyllene (19.5 %) and γ-muurolene (13.1%). The CO₂ supercritical extract was dominated by (E)-caryophyllene (26.0%), γ-muurolene (22.0%), and caryophyllene oxide (8.1%).