VEGETOS: Um Jornal Internacional de Pesquisa Vegetal

Chloroplast trnD-trnT Region Sequencing for Quick Haplotyping of Oak Populations

Josef Vlasak, Helena Cvrckova, Pavlina Machova and Jana Mala

Chloroplast trnD-trnT Region Sequencing for Quick Haplotyping of Oak Populations

Method of DNA isolation from young oak leaves is described that enables efficient amplification of trnD-trnT region of chloroplast DNA and direct sequencing of crude PCR reaction mixture. Sequence data of trnD-trnT region have been used to examine Quercus robur and Q. petraea population’s variability. High discrimination power of this method is demonstrated, comparable with previously used restriction analysis of four similar chloroplast DNA regions. Variable positions in trnT -trnD chloroplast DNA fragment are mapped and their use for oak haplotyping indicated..