VEGETOS: Um Jornal Internacional de Pesquisa Vegetal

Quick Detection and Diagnosis of Chilli Fruit Rot Pathogens

Santoshreddy Machenahalli, V B Nargund and Suresh Patil

Quick Detection and Diagnosis of Chilli Fruit Rot Pathogens

Chilli (Capsicum annuum L..), an important economic crop worldwide, is severely infected by fruit rot which may cause yield losses of up to 50%. The disease has been reported in all the chilli producing regions of the world and has become a serious constraint in chilli production. Different species of Colletotrichum, (C. capsici, C. gloeosporioides, C. acutatum), Alternaria alternata and Fusarium spp. are known to cause fruit rot in chilli. In the present study detection of pathogens from different plant parts was carried out by standard isolation method and PCR was done by using specific primers. The C. capsici was amplified as single band at 450bp whereas C. gloeosporioides and C. acutatum were amplified at 450 and 490bp respectively, Alternaria alternata amplified at 390bp and Fusarium sp. amplified at 550 – 570bp.