Jornal de Ciência Veterinária e Diagnóstico Médico

Development of VP6 Gene Specific Reverse Transcription(RT)-PCR Assay for Detectionof Avian Group D Rotavirus in Diarrheic Chickens

Jobin Jose Kattoor, Yashpal Singh Malik, Naveen Kumar, Kuldeep Sharma, Shubhankar Sircar, Munish Batra, Kuldeep Dhama and Raj Kumar Singh

 Development of VP6 Gene Specific Reverse Transcription(RT)-PCR Assay for Detection of Avian Group D Rotavirus in Diarrheic Chickens

The group D rotavirus (RVD) is an evolving viral cause of acute gastroenteritis in avian species world over. Despite the increase in the frequency of avian RVD infections world over, isolates from Indian poultry have yet to be characterised. Paucity of epidemiological studies with unavailability of sensitive and rapid detection methods for RVD in poultry led to design of the present study, aiming at development and validation of a RT-PCR assay, targeting the conserved group specific region of VP6 gene of RVD, for virus detection purpose.