Jornal de Ciência Veterinária e Diagnóstico Médico

Utilization of Denaturing Gradient Gel Electrophoresis (DGGE) to evaluate the Intestinal Microbiota of Brown Trout Salmo trutta fario

Marisa Manzano, Lucilla Iacumin, Cristina Giusto, Francesca Cecchini, Chiara Patthey, Ramon Fontanillas and Giuseppe Comi

Utilization of Denaturing Gradient Gel Electrophoresis (DGGE) to evaluate the Intestinal Microbiota of Brown Trout Salmo trutta fario

The microbial flora present in the intestine of the fish plays an important role, such as to break down ingested foods, or the inhibition of the colonization of the fish intestine by pathogens. Because only a small percentage of microorganisms in seawater and freshwater can be cultured and cultivated in the laboratory, molecular techniques based on 16S rDNA amplification have been developed to detect and identify culturable and nonculturable bacteria. Many studies have combined traditional methods with molecular methods to provide more detailed informations on microbial communities. The aim of this work was to evaluate the microbiota of Salmo trutta fario intestine, using PCR-Denaturing Gradient Gel Electrophoresis (DGGE), to assess the influence of a diet on the population composition.